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CABRI:ICLC |
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Accession_number |
ICLC HTL95007
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Cell_line_name |
A-704
|
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Brief_description |
Species: human; Tissue: kidney; Tumor: adenocarcinoma
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Description |
Species: human male 78 years old; Tissue: kidney; Tumor: adenocarcinoma
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Depositor |
Obtained from ECACC.
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Bibliographic_reference |
J Natl Cancer Inst 1973;51:1417-1423 [PMID: 4357758
] |
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Morphology |
epithelial-like, grown as monolayer
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Culture_conditions |
continuous culture; MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x105 cells/cm2. 37C, 5% CO2.
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Viruses |
Search for viruses was not performed
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Properties |
chemotherapeutic agents; cytotoxicity; tumorigenicity
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Passage_number |
53
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Species_validation |
Validated by isoenzymes: confirmed as human with MD, MPI
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Tumorigenicity |
non tumorigenic
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Karyology |
diploid to hyperdiploid
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Further_bibliography |
Oncogene 1994;9(3):939-942 [PMID: 8108138
]
Proc Natl Acad Sci USA 1987;84(22):7972-7976 [PMID: 2825172
]
|